Reproductive and genetic procedures for fish biodiversity and aquaculture conservation  


Head of the research program: Prof. Dipl.-Ing. Martin Flajšhans, Dr.rer.agr.

The research team of the RP 1 consists of selected employees from 6 scientific laboratories of the FFPW USB, namely the Laboratory of Molecular, Cellular and Quantitative Genetics (LMCQG), the Laboratory of Reproductive Physiology (LRP), the Laboratory of Germ Cells (LGC), the Laboratory of Intensive Aquaculture (LIA), the Laboratory of Lipid Metabolism (LLM) and the Laboratory of Signal and Image Processing (LSIP). Both faculty workplaces, the Genetic Fisheries Center (GFC) and the Experimental Fish Culture and Facility (EFCF) represent the operating base.



The present-day fish reproductive physiology and genetics are modern scientific disciplines which are generally applicable from sustaining and scientifically supported conservation of biological diversity over protection of aquatic genetic resources sensu FAO, i.e. significant for world agriculture and nutrition, to production of individual aquaculture commodities. A common key to them is a reproductive success based on production of physiologically and genetically high-quality gametes and their interactions both mutually and with external environment. Knowledge of these interactions in cartilaginous and bony fish has come to the fore of current scientific interest much like clarification of causes and consequences of gametogenesis disorders, including impacts on offspring generations and their reproductive capability. A modern trend in biological diversity conservation has also been represented by the development and creation of gene banks of living organisms and their cryopreserved gametes, but also banks enabling an international exchange and transfer of DNA, i.e. banks of tissues, embryos and germline stem cells. It is particularly the development of sturgeon germline stem cells bioengineering in which the FFPW USB team has belonged to world leading scientific groups and we would like to continue in the development of this field and also to develop GSC transfer methods with application to endangered or economically important species. Current concept of a breeding work in aquaculture, for example, possibility of using the selection according to genetic markers with a relation to required qualities (MAS - marker assisted selection), search for loci with a relation to quantitative characteristics (QTL - quantitative trait loci), application of indirect selection methods by a non-invasive method and mapping of a genetic structure of populations in order to search for suitable parental lines for creation of hybrids has also been based on application of molecular biology principles. On the basis of a scientific research and a pilot verification, we wish to efficiently pass on the discovered information to the production sphere.
The vision of the research programme is the biodiversity conservation and development of a competitive freshwater aquaculture through application of a multi-disciplinary approach on the basis of a synthesis of findings of existing scientific and research directions developed at individual workplaces of the Center. It involves development of a reproductive physiology and technology, molecular, cellular, quantitative and conservation genetics of individual species or higher taxa, development of reproductive technologies and germline stem cells bioengineering, their improvement by means of new approaches and development of new methods.
Objective of the RP
The objective of the RP is to ensure sustainability and development of excellence of the research and transfer of knowledge in the spheres of fish genetics, reproductive physiology and biotechnology into practice in order to sustain fish biodiversity, establish an internationally acknowledged gene bank and improve competitiveness of the European aquaculture with a significant share of the Czech Republic. Five domains of an excellent research of reproductive and genetic procedures have been delimited out of these which are considered to be essential for sustaining fish biodiversity and for the development of Czech and European aquaculture. These domains are: research of fish gametes and their interactions during the fertilization process; study of gametogenesis disorders, biology of such emerged polyploid organisms and optimization of reproduction biotechnology; optimization of technologies and protocols for the international gene bank; germline stem cells bioengineering and application of principles of molecular biology in fish breeding. An integral part of individual partial objectives will be management and data mining.
The outcomes of experiments and studies achieved by the research team have been regularly published and an emphasis has been put on application of the outcomes in prestigious journals. Since 2010, members of the research team have contributed to publishing 207 articles in impacted journals, 2 scientific monographs, 10 chapters in books, 19 certified methodologies and 16 technologies, 2 utility models and 1 acknowledged breed. An important activity of the research team is also commercial activities generating significant financial resources. Since 2010, the research team laboratories have implemented contracts in the total volume of almost CZK 10.3 million and an operating base of the team (GFC and EFCF) in the volume of CZK 12 million. Members of the research team have also succeeded in obtaining and solving research projects. At present, we have been researchers/co-researchers of 10 international research projects (5x 7th RP EU and Horizon 2020 programme, 2x COST and 3x EEA and Norway Grants – Bilateral Scholarship Programme CZ07).
rybarskeknihy   youtube  face

© 2014 Fakulta rybářství a ochrany vod ve Vodňanech