I spent three weeks (11th – 29th May) in “Leibniz-Intitut für Zoo und Wildtierforschung“, Berlin. During these days I was working under supervision of dr. Arne Ludwig and Anke Schmidt. I have learned techniques for Next Generation Sequencing methods, i.e. extraction of RNA, revers PCR for cDNA preparation, cDNA preparation and purification and running of Illumina MiSeq transcriptome sequencing of Procambarus fallax f. virginalis. Next month I will spend by analysing huge amount of data coming from the Illumina MiSeq runs. This stay was allowed due to financial support from Postdok II project (CZ.1.07/2.3.00/30.0049).