FROV JU RGB NEGATIVE
 
Staff and contacts
 
velisek
Assoc. Prof. Dipl.-Ing. Josef Velíšek, Ph.D.
head of laboratory, academic worker
+420 38777 4625     +420 725 787 931
                                   
steinbach 
Dipl. Biol. Christoph Steinbach, Ph.D.
research scientist
steinbach@frov.jcu.cz
+420 38777 4622
             
machova
Dipl.-Ing. Jana Máchová, Ph.D.
research scientist
+420 38777 4754
 
 
 
 
 
             
kroupova
Dipl.-Ing. Hana Kocour Kroupová, Ph.D.
head deputy, academic worker
+420 38777 4621
 
 
lidova 
Dipl.-Ing. Jaroslava Lidová
External PhD student
publication
 
             
svobodova
Prof. Zdeňka Svobodová, MVD., DSc.
research scientist
+420 38777 4625
 
koutnik
Dipl.-Ing. Dalibor Koutnik
External PhD student
 
             
valentova
Dipl.-Ing. Olga Valentová
academic worker
+420 38777 4754
 
 chupani
MSc. Latifeh Chupani, Ph.D.
research scientist
lchupani@frov.jcu.cz
+420 38777 4715
             
zuskova
Eliška Zusková, MVD., Ph.D.
academic worker
+420 38777 4621
 
sauer 
Dipl.-Ing. Pavel Šauer
PhD student
psauer@frov.jcu.cz
+420 38777 4714
curriculum vitae CS
curriculum vitae EN
publication
             
piackova
Veronika Piačková, MVD., Ph.D.
academic worker
+420 38777 4621
 
priborsky 
Dipl.-Ing. Josef Příborský
external PhD student
priborsky@frov.jcu.cz
+420 38777 4763
             
 mohammadi
MSc. Mohammadi Mehrak
doctorand
+420 38777 4714
publication
 
prokopova
Ilona Prokopová
technician
prokopova@frov.jcu.cz
+420 38777 4754

 

             
 stara
Dipl.-Ing. Alžběta Stará, Ph.D.
academic worker
+420 38777 4622
 
 
Dipl. - Ing. Marie Šandová
technician
msandova@frov.jcu.cz
+420 38777 4625
curriculum vitae EN 
             
 

Laboratory unites experts in the field of Veterinary Medicine, Aquatic Toxicology, Hydrochemistry and Biochemistry, who are engaged in basic and applied research, university teaching, and consulting and advisory activities. Laboratory deals with the research programs of centre CENAKVA: WP 3 Innovation of intensive production methods of economically important fish species and species important for angling and WP 5 Innovation in monitoring the occurrence of extraneous substances in environment, evaluation of the influence on exposed organisms and possibilities of elimination in clearing processes.


The wokplace toxicology and hydrochemistry are important parts of this laboratory.

On workplace toxicology, acute toxicity test on fish, daphnia, freshwater green algae, and white mustard seeds are routinely performed. On the basis of obtained data, ecotoxicological properties of substances, chemical products and wastes are assessed. Moreover, for scientific purposes are performed sub-chronic and chronic toxicity tests on fish, crayfish and their early-life stages.
 

32akvarijka  
 
Toxicological aquarium room (left), Toxicity test on roach (right)
 

Workplace hydrochemistry performs analysis of basic parameters of water quality for research institutes and fish farmers. Every year laboratory successfully participates in inter-laboratory comparison tests organized by the Centre for Assessment of Laboratories ASLAB at T. G. Masaryk Water Research Institute, public research institution (TGM WRI) in Prague and obtain a certificate of participation. In this way, continual external control of both laboratories is guaranteed.
Staff of the laboratory also provides manufacturing and service of Combi sets which are used for field analysis of water. Moreover carries out analysis of total mercury in biological materials and sediments.

 
Combi set
 
Basic and applied research of the Laboratory of Aquatic Toxicology and Ichtyopatology is focused on the three main areas:

Study of influence of xenobiotics on aquatic organisms
Mainly studies aimed at evaluating the effects and mode of action of xenobiotics (e.g. pesticides, drugs, nitrite, cyanide) on physiology of fish and crayfish are carried out. Recently the laboratory is focused on monitoring the occurrence of endocrine disrupting chemicals, especially synthetic progestin’s in the aquatic environment, and assess their effects on fish. The effect of xenobiotics present in the aquatic environment on aquatic organisms is mainly assessed by analysis of selected haematological and biochemical indices, activity of detoxication and antioxidant enzymes, gene expressionand histopatological assessment of tissues.
Workplace is equipped for these purposes with modern devices such as: double-beam spectrophotometer SPECORD 210, plate-reader with fluorescent and luminescent module Infinite M200PRO (TECAN), biochemical analyzer of blood plasma VETTEST, analyzer of blood gases Radiometr ABL90 Flex, biohazard flow-box, real-time PCR thermocycler MX3005P Agilent, PCR thermocycler with temperature gradient Biometra, 2100 Bioanalyzer and micro-volume UV-Vis spectrophotometer NanoDrop 2000.  In 2013, workplace was equipped with complete facilities for the preparation of histological sections (tissue processor Histomaster (Model 2052/1,5), modular tissue embedding center (Leica EG1150), semi-automatic rotary Microtome, automatic slide staining system (TISSUE-TEK® DRS™ 2000, SEKURA) and microscopy combined with camera system MOTIC).
 
 
Common carp after triazine exposure
 
  
 
Blood taking of the Siberian sturgeon (left), field sampling (right)
 

Prevention and therapy of fish diseases
This is mainly focused on verification and the development of strategies in the treatment of the most common diseases in aquaculture. Therapeutical and rearing procedures aimed at minimizing of losses caused by various pathogens are designed for fish farmers. At present attention is mainly paid to the possibility of using peracetic acid in the aquaculture, including verification of spectrum efficiency and application possibilities. Results of laboratory and field experiments were used for preparation of methodology entitled „Possibilities of using peracetic acid in the aquaculture ˮ.
The koi herpes virus (KHV) is an important issue that laboratory staff is engaged in. In cooperation with the Genetic Fisheries Centre of the faculty (FFPW) and Veterinary Research Institute (VRI Brno), testing of resistance of pure breeds and hybrids of common carp to KHV under laboratory conditions are performed. The aim is to identify breeds and hybrids, which have showed the highest survival in experimental infections, to expand their use in the production of fisheries and thus contribute to reduction of the risk of occurring and spreading of koi herpes virus in the Czech pond aquaculture.
Moreover, staff of the laboratory performs basic pathological and parasitological examinations of freshwater and aquarium fish for fish farmers.

  
 
Parasitological investigation of fish (left), determination of intensity of mold Saprolegnia parasitica infection in brook trout (right)
 
Monitoring the impact of aquaculture management on water quality in ponds
The staff of laboratory solved the project NAAR entitled „Environment friendly and effective pond management with maximal utilization of current trophic potential and sustainable good water quality and fish production“, focused on monitoring water quality in ponds in relation to the method of fish farming. The aim of project was to answer the question whether it is possible meet the water quality parameters (pollution standards) in ponds.
 
Indicator of water quality The amount of indicator standard obr vylov
pH 6 – 8
O2 (mg.l-1) min. 6
Biological oxygen demand BOD (mg.l-1) 6
Chemical oxygen demand CODCr (mg.l-1) 35
Total phosphorus
Ptotal (mg.l-1)
0,20
Total nitrogen Ntotal (mg.l-1) 8
Ammonium nitrogen
N-NH4 (mg.l-1)
0,5
Nitrite nitrogen
N-NO2 (mg.l-1)
0,14
Nitrate nitrogen
N-NO3 (mg.l-1)   
7
Suspended solids SS (mg.l-1) 30
 

Results of five years monitoring showed that very problematic appears respect BOD value, that has been exceeded in more than 50% even in cases of very low initial stocking without feeding. In contrast, as the no problem appeared concentrations of total nitrogen and his forms. Water quality parameters such as CODCr, total phosphorus and suspended solids are negatively affected by the level of stocking and feed applications. The proportion of unsatisfactory values of different water quality parameters in relation to the initial stocking of fish are shown in the following graphs:

 
 
 
The staff of laboratory in collaboration with colleagues from the Veterinary and Pharmaceutical University Brno published a book Aquatic Toxicology for Fishermen in 2014. This book summarizes the knowledge about pollutants in aquatic environment and their effect on aquatic organisms, toxicity tests on aquatic organisms; investigation fish kill poisons and natural toxin.
 vodni toxikologie

Book Velíšek, J., Svobodová, Z., Blahová, a kol., 2014. Vodní toxikologie pro rybáře. FROV JU, Vodňany, 600 s, ISSN 978-80-87437-89-6.


Staff of the laboratory cooperates also with Police of the Czech Republic, fishery associations and municipal authorities on the investigation of the causes of acute fish kills. Moreover, the laboratory monitors effects of pesticides on non-target aquatic organisms and records the acute fish kills in the Czech Republic in cooperation with the Ministry of Agriculture.

 


 
 
 

 

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