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Staff and contacts
 
boryshpolets
Mgr. Sergii Boryshpolets, Ph.D.
head of laboratory, academic worker
+420 38777 4615
publication
     
rodina
Dipl.-Ing. Marek Rodina, Ph.D.
head deputy, academic worker
+420 38777 4614
publication
     
linhart Prof. Dipl.-Ing. Otomar Linhart, DSc.
akademic worker
+420 724 357 897, +420 38777 4600
     
 cosson
Jacky Cosson, Ph.D., Dr.h.c.
academic worker
+420 38777 4615
     
 dzyuba b
MSc. Borys Dzyuba, Ph.D.
academic worker
+420 38777 4614
     
dzyuba v MSc. Viktoryia Dzyuba, Ph.D.
research scientist
+420 38777 4614
 
 
 
bondarenko o
MSc. Olga Bondarenko
research scientist
+420 38777 4607
     
siddique MSc. Mohammad Abdul Momin Siddique, Ph.D.
research scientist
+420 38777 4748
 
 
 
li p
MSc. Ping Li, Ph.D.
academic worker
curriculum vitae CS
     
horokhovatskyi
MSc. Yevhen Horokhovatskyi
PhD student
+420 38777 4615
curriculum vitae CS
curriculum vitae EN
publication
 
 
 
dadras
MSc. Hadiseh Dadras Asyabar
PhD student
+420 38777 4743
curriculum vitae CS
curriculum vitae EN
publication
 
 
 
tuckova
Mgr. Vladimíra Tučková
laboratory technician
+420 38777 4717
curriculum vitae CS
curriculum vitae EN
publication
     
xin
MSc. Xin MiaoMiao
PhD student
+420 38777 4743
curriculum vitae EN
publication
     
kholodnyy
MSc. Vitaliy Kholodnyy
PhD student
curriculum vitae EN
publication
     
bondarenko v
Volodymyr Bondarenko, MSc., Ph.D.
Worker in Biological Sciences
+420 38777 4717; +420 724 504 922
 
     
shelton
prof. MSc. William L. Shelton, Ph.D.
mentor
curriculum vitae EN
publication
 

The laboratory carries out besides lecturing also basic and applied research with focus on reproduction of our economically significant and endangered fish species primarily.
The main tools are microscopes equipped with stroboscopic lamp, high speed camera and computer assisted system and software of image analyses for evaluation of sperm motility parameters, bioluminescent method for ATP measuring, proteomics, micromanipulations, fluorescent, confocal and electron microscopy. Tests of fertilization and hatching are performed at Genetic fisheries center. The outputs are in form of methodologies, technologies and research articles focused on activation, reactivation and immobilization of gametes, sex determination, determination of maturity stages in females, genomic manipulations, short and long term storage of gametes (cryoconservation), sex reversal and evaluation of endocrine disruptor effects. The laboratory possesses a gen bank with cryopreserved sperm, blastomers and spermatogonia of various fish species and breeds. Laboratory deals with the research program of centre CENAKVA RP 2 Biology, conservation and aquaculture of sturgeons.

The laboratory also offers assistance in sex and maturity stage determination and in artificial reproduction of fish itself, especially in sturgeons.

 
The description of rotation of sterlet spermatozoa flagella on the bottom of drop. Time between frames is 2 millisec. This picture is from study of Sergey Boryshpolets, which gained the award for the best presentation of PhD student during the 3rd International Workshop on the Biology of Fish Gametes in Budapest, Hungary.
 

    
The acrosome of Siberian sturgeon before and after activation.
 
 

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Freezing of fish sperm and embryos
A)  work on freezing machine freezing the carp sperm;
B)  freezing machine for cell freezing to -196 °C;
C) gutters with individual boxes, heating, recirculation and UV sterilization are used for testing fertilization and hatching performance.
 
 
        
The determination of sex and oocyte stage using biopsy and collection of eggs in sterlet.
 
 The hermaphroditic carp gonad with spermatocytes and oocyte

 
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Laboratory has organized the first international workshop with the topic of fish sperm biology with participation of 13 states from four continents in August 2007. Lectures and poster message have been published in the Journal of Apply Ichtyology, 24 (5), (2008).

 

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