/CENAKVA/

South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses

Large research infrastructure CENAKVA

vvi_services      open-access vvi_performed-projects   vvi_open-data

The aim of the large research infrastructure CENAKVA is an understanding of changes in freshwater ecosystems and their social importance in terms of biodiversity conservation, protection of the aquatic environment and water resources important for human life and activity. Large research infrastructure CENAKVA covers infrastructure, knowledge and expertise of the Faculty of Fisheries and Protection of Waters a South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses and enables open access to the 25% of the capacity.

Large research infrastructure CENAKVA operates on the principle of open access infrastructure for experimental activities, which includes a total of 12 specific laboratories and research workplaces.
Unique pond, experimental and scientific background together with close ties to the fishing community in the Czech Republic, Europe and the world, which CENAKVA has, creates a unique unit, which is able to plan and verify future proposals for management of ponds, due to climate changes in the Czech Republic and Europe.

Large research infrastructure belongs to the field of environmental sciences Roadmap of the Czech Republic for large infrastructures for research, experimental development and innovation. In the future, the main task of fishermen will not be to produce fish, but to maintain quality water in a quality landscape, creating a regional climate that reduces drought and flooding.

Feel free to sign up with your experiment! The call for projects is open continuously!

The applicant shall complete the Form electronically with a structured curriculum vitae and wait for the project to be evaluated and decide whether the project will be implemented.

CENAKVA LRI - Summary information

Infrastructure and services

Experimental Fish Culture and Facility
 Experimental Fish Culture and Facility
12 x circle tanks in flow system (1500l) – for experiments in outside conditions 01 ERPP01
6 tanks in recirculation system with control conditions (800l) – for experiments focused mainly on the research of breeding technologies in Aquaculture 01 ERPP02
Laboratory of Environmental Chemistry and Biochemistry (LECHB)
 Laboratory of Environmental Chemistry and Biochemistry
Two-dimensional liquid chromatograph with high resolution mass spectrometer (QExactive) – analytical instrument used for targeted analysis of pharmaceuticals, pesticides, hormones, UV filters, perfluoronated substances and other micropollutants mainly in biological samples of aquatic organisms and plants 02 LECHB01
Two-dimensional liquid chromatograph with high resolution mass spectrometer (QExactive-HF) – analytical instrument for targeted and untargeted screening of aquatic environment 02 LECHB02
Two-dimensional liquid chromatograph with triple quadrupole mass spectrometer (Quantiva) – analytical instrument used for quantitative determination of pharmaceuticals and pesticides in water, passive samplers, sediments, sludge and soils 02 LECHB03

LC-QqQ-MS TSQ Altis is a triple quadrupole mass spectrometer suitable for quantitative  analysis on residual concentration levels of compounds; Ion source allows the use of heated electrospray or chemical ionization; A two-dimensional liquid chromatography system allows online solid-phase extraction   suitable for residual analysis of pollutants in the water sample matrix; The Autosampler unit has a capacity of up to 324 samples; Analysis of 109 pharmaceuticals, 110 pesticides, and their metabolites; Sample types- soil, sludge and passive sampler extracts, water matrix;Limits of quantification- ng/mL (extracts), ng/L waterody.

Benefits for Ph.D. students using the facility

altis
Laboratory of controlled reproduction and intensive fish culture (LRICH)
Laboratory of controlled reproduction and intensive fish culture 
Experimental recirculating aquaculture system for growing trials on fish. Fully equipped modular experimental system is available. 03 LRICH01
Self-feeding system for feed distribution (dosing of precise amount of feed) and evaluation of feed demands. Software allow creating of feeding protocols and evaluation of feeding behaviour. Software control culture on three levels (overview about reared fish, evolution in time and detail in each culture unit). 03 LRICH02
Complete system of swimming tunnels / respirometers for computer assisted intermited respirometry. The system allows fully automated measurement of oxygen consumption, evaluation of the critical swimming speed (Ucrit) and fish behavior. For research focused on biomechanics, physiology of swimming, metabolism and ethological studies. 03 LRICH03
Laboratory of experimental complex systems (LECOS)
 Laboratory of experimental complex systems
Tissue cultures laboratory, animal cell dynamics observation. Tissure culture laboratory has nearly 20 years tradition in services for private, clinical an university laboratories. It is concerned with obsarvation of dynamics of mammalian cells in interactions with materials used in the medicine. 04 LEKS01
Superresolution microscopes NanoTrutht. The NanoTruth technology utilises long term known techniques wich may me fully utilised only using contemporary technologies. For data analysis there are used new discrete mathematics approaches developer by the researchers of the LECOS laboratories. New NanoTruth microscope prototypes were finalised in 2019 and are the final phase before possible industrial production. 04 LEKS02
5iD viewer – complex analysis of the fish school behavious down to the role of the individuals. The 5iD viewer is the word first instrument enabling the fish school analysis down to the level of an individual. The key are new algorithms which identify each individual and prevent error propagation. 5iD viewer, besides basic research, opens the possibility to mediciene research practice, where it minimises the number of test animals, water cleaning station practice, veterinary practice and many other applications. 04 LEKS03
Laboratory of Reproductive Physiology (LRP)
 Laboratory of Reproductive Physiology
Evaluation of sperm quality and motility (including flagellar motion) before and after cryopreservation. Involves several microscopes connected to digital and analog video cameras, covers different contrast methods (BF; DF; PH), temperature diapason (4 - 37°C), frame rate (5 – 5000 FPS), magnifications (up to x100) and resolutions (up to 3200×1800) during motility records. 05 LFR01
Laboratory of Aquatic Toxicology and Ichtyopathology (LVTI)
 Laboratory of Aquatic Toxicology and Ichtyopathology
Gene expression analysis and other biochemical analyses. Equipment: Real-Time PCR cycler, Bioanalyzer, Nanodrop, Infinite M200PRO fluorescence and luminescence spectrophotometer (TECAN), SPECORD two-beam spectrometer. 06 LVTI01
Histopathological examination of tissues of aquatic organisms - is based on a complete device for the preparation and analysis of histological preparations of tissues of organisms. Equipment: Histomaster dewatering machine (Model 2052 / 1.5), modular potting paraffin line (Leica EG1150), semi-automatic rotary microtome, automatic coloring line (TISSUE-TEK® DRS ™ 2000, SEKURA) and MOTIC microscope. 06 LVTI02
Laboratory of intenzive aquaculture (LIA)
 Laboratory of Intensive Aquaculture
Two identical experimental rooms for reproduction and intensive aquaculture. In each room there are nine 360 L tanks connected to the complete RAS with the possibility to control the light and temperature regime. Culture systems can also be used for experiments related to the final reproduction phase of fish, culture of juvenile fish under different stocking densities, feeding management using self-feeders and different dissolved oxygen level. 07 LIA01
The fully acclimatized room is fully equipped for intensive culture of cold-water fish (salmonids and whitefish species) and for stimulation of out-off-season spawning of various fish species. This room contains four 1500 L tanks and six 360 L tanks connected to a complete RAS. 07 LIA02

Experimental hall for intensive aquaculture with a total capacity of twenty 1500 L tanks connected to two separate RAS. This equipment can be used for mass scale experiments with the aim to optimize intensive aquaculture in terms of water quality, feed management, nutrition, stocking density, fish sorting, ozone using, etc

07 LIA03
Laboratory of Molecular, Celullar and Quantitative Genetics (LMBKG)
 Laboratory of Molecular, Cellular and Quantitative Genetics
Applied Biosystems 3500 and Genetic Analyzer Next generation sequencer Ion Torrent™ PGM for sequencing of mitochondrial and nuclear genes. Gene sequencing is used especially to parentage assignment, to identify monosex fish stocks and to study the mutual genetic and molecular biological status of fish and crustacean populations. 08 LMBKG01
Flow cytometers Partec CCA-I and CyFlow® Cube 8 are used especially for determination of the ploidy level on the basis of relative DNA content in nuclei of fish somatic cells or gametes stained with DNA-specific dyes. Another possibility is the stipulation of volume and concentration of measured particles.
Laboratory of germ cells (LGC)
 Laboratory of Germ Cells
Zebrafish housing system – semiautomatic system for rearing of small fishes and for initiation of feeding of fish larvae. 09 LZB01
System for fish embryo injection and micromanipulation – it is suitable for all fish species from early embryonal stages to larval stages. 09 LZB02
DNA content analyser – user friendly flow cytometer enables evaluation of relative DNA content in the cell nuclei 09 LZB03
Capillary electrophoresis – electrophoretic system with resolution under three bases is suitable especially for more precise determination of PCR product size. 09 LZB04
Laboratory of signal a image processing (LSIP)
 Laboratory of signal and image processing
Monitoring systems for fish ethological studies - based on 3D cameras allow automated recording and evaluation of fish behavior in aquariums and tanks 10 LZSO01
Hyperspectral camera - Scanning and analyzing samples using hyperspectral camera, surface reflectivity analysis of materials that are subsequently correlated with substance content or material composition, typical task is analysis of fish appearance using different diets, scanning camera allows processing of any materials - visible + near infrared spectrum 10 LZSO02
Laboratory of Nutrition (LV)
 Laboratory of Nutrition
Aquaponics hall contains 6 independent recirculating aquaculture systems with waste water collection and mineralization. 200 square meters of greenhouse with 4 types (raft, ebb and flow, dripping and aeroponic systems) of hydroponic systems (each with at least 6 replicates). 11 LV 01
Chromatography lab including sample preparation room for complex analyzes of feeds, fish products, feaces and other matrices. Available instuments are: GC-MS, HPLC, HPTLC, Maldi-tof. 11 LV 02
Fully equipped fish processing plant for production and development of fish products (chilled, frozen, smoked, cooked, marinated), including analytical background for studies of postmortem changes in fish and assessment of fish product quality. 11 LV 03
Laboratory of freshwater ecosystems (LFE)
 Laboratory of Freshwater Ecosystems
EthoVision® XT set-up – ethological software with equipment (hardware, video-cameras, experimental arenas) in the ethological laboratory. Enables observation of aquatic organisms and automatic evaluation of their behavioural patterns. Possibility of simultaneous observation up to 96 arenas. 12 LSE 01
Simulator of flow conditions for fish and crayfish. Enables to mimic the conditions of running waters for experimental observation of fish and crayfish. 12 LSE 02
Aquarium room. Infrastructure used for maintenance and culture of crayfish and for experimental set-ups. 12 LSE 03
The set of incubators in the hydrobiology laboratory. Enables maintenance of aquatic organisms and experimental works in temperature specific conditions (4 – 40°C). 12 LSE 04
Heart rate monitoring system for crayfish and bivalves. Non-invasive measurement of heart rate of crayfish and bivalves under laboratory conditions. 12 LSE 05
Facilities for rearing and experimental maintenance fish or crayfish (outside and inside systems of tanks). Maintenance and culture of fish and crayfish and for experimental work under natural ambient as well controlled conditions (including the possibility of the water recirculation). 12 LSE 06
DanioVision® set-up with equipment in separated laboratory. System enables observation of development and life manifestations (including heart rate activity) of aquatic organisms during embryonal and postembryonal development under controlled light and temperature condiditons. 12 LSE 07
Sampling of water and hydrobionts (fish, zoobentos, zooplankton, phytoplankton) in freshwaters (pond and their tributaries, different types of streams and rivers, lakes), measurements of physical and chemical parameters of water, samples processing and analysis. 12 LSE 08
Facility for ecological and ethological studies of hydrobionts, observation of their foraging behaviour (mainly fish and macrozoobenthos), experimental works on zooplankton and phytoplankton, and early developmental stages of fish, and for evaluation of approaches and preparations for artificial reproduction of fish. 12 LSE 09

Laboratories in cooperation

Laboratory of the Faculty of Agriculture USB

Department of Agricultural, Transport and Handling Technology

Studentská 1668, 370 05 České Budějovice

Photoacoustic Gas Monitor – Innova 1512

Available from 01/2022

Benefits for Ph.D. students using the facility

 

Photoacoustic Gas Monitor – Innova 1512 can simultaneously monitor the concentration of CO2, NH3, and water vapour in any air sample based on the principle of photoacoustic infrared spectroscopy. The device is well suited and very efficient in both short and long-term monitoring applications. In short-term monitoring, the benefit is portability, minimal warm-up time, and built-in data storage capability. In long-term monitoring, the system is especially stable, includes a multi-point sampling option and data handling.  The device is equipped with a converter capable of taking samples from up to 12 different locations and can operate in difficult microclimatic conditions such as those in livestock farms. The detection limit for CO2 is 13 ppm, and for NH3 is 0.20 ppm.

 

Contact:

Dipl.-Ing. Radim Kuneš

E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Phone: +420 606 263 674

analyzator-plynu
Laboratory of the Faculty of Science USB

Laboratory of Molecular Integrative Physiology in Drosophila,

Branišovská 1760, 370 05 České Budějovice

High-resolution 3D X-ray microscope

Available form 02/2022

Benefits for Ph.D. students using the facility

 

Visualization of complex internal structures of biological objects without the need for their disintegration by fixation and tissue section is one of the challenges of modern biology. To overcome this limitation, we will soon operate the High-resolution 3D X-ray microscope. A series of hundreds of X-rays of a rotating object enables the subsequent 3D reconstruction of objects and their internal structures with a resolution of fewer than five μm. The device can be used to scan various objects from plants, stones, composite materials, building materials, and even live animals. The combination of natural contrast properties of biological structures with specific contrast agents and antibodies provides an infinite potential for implementing this tool in research carried out not only at the Faculty of Science. Furthermore, compact layout, absolute safety, and simple operation allow independent work of doctoral students.

 

Contact:

Mgr. Adam Bajgar, Ph.D.

E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Phone:  +420 387 772 227

                                                                                                                                               
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Open DATA

 

For open/shared data services, please contact the person responsible for providing you with all the details.

Responsible person: Jaroslava Dubová, BBus.

E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

The data request form is available here.

Project application process

  • The call for access is opened continuously
  • The applicant fills in the external internal form for access
    • Internal access - the employees of University of South Bohemia in České Budějovice
    • External access - others
  • Send the form together with the applicant’s CV to the call manager: Jaroslava Dubová - This email address is being protected from spambots. You need JavaScript enabled to view it.– the subject of the email is OPEN ACCESS
  • Wait for the decision of the proposal evaluation (14 days after call deadline)
  • In the case of project acceptance:
    • Call manager will send you the agreement to sing – send signed document back to call manager
    • Agree with the head of the laboratory on the project implementation

Open access - How to sign up

Performed projects

Selected open acces projects of LRI

Comparative performance experiment of Silurus glanis strains and families

Lead researcher: Prof. Riho Gross

Project period: 03.06.2019 / 21.06.2019

Laboratory: Laboratory of molecular, cellular and quantitative genetics

Project No: 2019-02-22

Project was focused on collection of tissues samples of wels catfish broodstock of strains established at the Genetic Fisheries Centre of FFPW USB and characterization of genetic variance  within and between strains/families and for parentage assessment in families of their progeny using microsatellite genotyping and NGS. Following selection and qualitative assessment of reproductive performance, factorial mating of 6 females and 6 males per strain was used to establish full-sib and half-sib families. Following egg incubation and hatching, the fry was air freighted to Estonia for comparative evaluation of performance of the strains and families and to estimate heritability of commercially important traits.

Publications here: https://academic.oup.com/g3journal/article/10/11/3897/6048642

Obrzek__1

PBSA analysis in water samples

Lead researcher: Dr. Dipl.-Hyd. Jakob Schelker

Project period: 02.03.2020 / 24.03.2020

Laboratory: Laboratory of Environmental Chemistry and Biochemistry

Project No: 2020-01-63

Streams and rivers metabolize dissolved organic matter (DOM). Although most DOM compounds originate from natural sources, recreational use of rivers increasingly introduces chemically distinct anthropogenic DOM. The aim of this study was firstly to investigate anthropogenic effect on the increase of DOM in Traisen River in Austria during music festival, and secondly to examine fluorescence spectroscopy for monitoring DOM. Sunscreens and especially their component Phenylbenzimidazole sulfonic acid (PBSA) was found as the main source of anthropogenic pollution. This was confirmed not only by the fluorescence method but also by liquid chromatography coupled with mass spectrometry analysis of water samples in Laboratory of Environmental Chemistry and Biochemistry.

Publications here: https://pubs.acs.org/doi/full/10.1021/acs.est.0c02259

obrazek-c_-2

Population-specific immune response in percid species

Lead researcher: Alexander Rebl, Ph.D.

Project period: 01. 08. 2020/ 31. 08. 2020

Laboratory: Laboratory of controlled reproduction and intensive fish culture

Project No: 2020-03-75

European perch, Perca fluviatilis, is a highly promising candidate for freshwater aquaculture diversification. It has great potential to deliver products of sensory quality and nutritional value according to the market demands in the EU. However, European perch production is often hampered by high sensitivity to stress under captive conditions which has a negative impact on reducing immune resistance and at the same time increasing sensitivity to diseases and pathogens. For the successful prevention of diseases, detailed information on the molecular mechanisms of immune responses among geographically distinct European perch populations against different pathogenic stimuli is requested. To overcome this obstacle, we established in vitro model of inflammation, using the stimulation of head kidney leukocytes with lipopolysaccharide (LPS), the major component of the outer membrane of Gram-negative bacteria. To this end, head kidney leukocytes (HKL) from four different European perch populations (France I and II, Denmark, Hungary) were isolated using density-gradient centrifugation and stimulated with LPS (100 µg/ml) for one hour. Following the incubation, the respiratory burst was measured using the dihydrorhodamine 123.

Publications here: https://www.researchgate.net/publication/346714561_DEVELOPMENT_OF_IN_VITRO_MODEL_FOR_STUDIES_OF_INFLAMMATORY_PATHWAY_IN_EUROPEAN_PERCH

obrazek-c_-3

Collagen from various mammal species as matrix for tissue engineering

Lead researcher: Ing. Lucie Vištejnová, Ph.D.

Project period: 01.11.2019/ 31.12.2019

Laboratory: Laboratory of experimental complex systems

Project No: 2019-04-53

One of the key responses of cells on collagen-based materials is the mechanism of their adhesion on the material surface and their locomotion and proliferation on it. Therefore, the objective of this project is to evaluate the adhesion, spreading and locomotion of cells on collagen coming from different species and extracted by different methods. To extract information about cell structure and dynamics we had to develop respective software for which we developed new statistical methods for evaluation of physical parameters. We are, as first in the world, able to determine spectra of individual organelles, local diffusion, local directed flow inside the living mammalian cell and many other parameters.

Publications here: https://www.nature.com/articles/s41598-020-75441-7

obrazek_kolagen

Sea bass identification - non-invasive approach

Lead researcher: Prof. Andreas Uhl

Project period: 23.11.2020/ 20.12.2020

Laboratory: Laboratory of signal and image processing

Project No: 2020-04-96

Precision fish farming is an emerging concept in aquaculture research and industry, which combines new technologies and data processing methods to enable data-based decision making in fish farming. The concept is based on the automated monitoring of fish, infrastructure and the environment, ideally by contactless methods. The identification of individual fish of the same species within the cultivated group is critical for individualized treatment, biomass estimation and fish state determination. The standard way of fish identification is invasive fih tagging. The project test the posibilities of long-term fish identifiaction based on the pictures of eye pattern. We sucessfully showed that the eye pattern can be used for the identification of individual fish and can be a non-ivansive substitute to the invasive fish tagging.

Publications here: https://ieeexplore.ieee.org/document/9133326

obrazek_bass

Use of probiotics for pikeperch (Sander lucioperca) mass production

Lead researcher: MSc. Astrid Sibylle Holzer, Ph.D

Project period : 01.06.2019/ 02.08.2019

Laboratory: Laboratory of Intensive Aquaculture

Project No: 2019-01-12

The aim of the project was to examine the application of artificially produced rotifers of the species Brachionus plicatilis as the first exogenous nutrition in intensively reared pikeperch larvae (Sander lucioperca). The study of the project examined a probiotic based on Pediococcus acidilactici lactic acid bacteria in Bactocell Aqua 100 preparation. This product was tested in the form of incorporated bacteria into the body of the rotifers or enriched water with bacteria that was used for larval culture. In second study of the project, the effect of enriching produced rotifers with unsaturated fatty acids (PUFA - EPA and DHA) was tested using artificially cultured Chlorela algae or additives like Selco, Spresso preparation from INVE company from Belgium. The aim of both studies was to increase the survival rate and physiological resistance of cultured larvae to produce high-quality juveniles  for further industrial farming.

Publications here: https://www.mdpi.com/2076-2607/8/2/238

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Implemented open access projects LRI

Implemented projects in 2021
2021
Project no
Name of the project
country of the solver
2020-03-79 Effect of elevated temeprature on trophic relationship of marbled crayfish in food webs of Hevíz lake and their chanels Hungary
2021-01-98 Israel Chemcatcher Screening UK
2021-01-99 Effects of dietary protein and soybean meals on growth perfromance, body composition, blood biochemistry in pikeperch (Sander lucioperca) Ukraine
2021-01-100 Optimization of the intensive rearing of rainbow trout (Oncorhynchus mykiss in RAS) Czech Republic
2021-01-101 Effect of sinking and floating feed in the rearing of European pike perch (Sander lucioperca) Czech Republic
2021-01-102 Analysis of macroergic phosphates and metabolites in sterlet spermatozoon Czech Republic
2021-01-103 Analysis of macroergic phosphates and organic acids in sperm of stingrays during storage Czech Republic
2021-01-104 Occurrence of (anti-)progestagenic activities in Czech wastewaters and surface waters Czech Republic
2021-01-105 Non-targeted screening and quantification of cortisol in stugeons´ plasma Czech Republic
2021-01-106 Phenotype modifications and functional properties during fish sperm aging Czech Republic
2021-01-107 Optimization of Sperm Management and Fertilization in Zebrafish [Danio rerio (Hamilton) Czech Republic
2021-01-108 Effect of anti-progestagenic activities on Xenopus leavis Germany
2021-02-109 Choosing the best diet for the aging fish model -turquoise killifish Czech Republic
2021-02-110 The cognitive ability of fish during food intake in a new environment after restocking Czech Republic
2021-02-111 Evaluation of different approaches for fish plasma sampling with respect to environmental risk assessment of pharmaceuticals Sweden
2021-02-112 Stress response and DNA damage in mosaic sturgeon (Acipenser ruthenus) larvae Czech Republic
2021-02-113 The effect of in-vitro oocyte ageing and highly concentrated sperm insemination on the incidence of spontaneous autopolyploidy and mosaicism in sterlet Czech Republic
2021-02-114 Behaviour and fitness in cultured asp (Leuciscus aspius) Czech Republic
2021-02-115 IN-VITRO CELL LINE CULTURE BEHAVIOUR AFTER IMPLICATION OF FULL-SPECTRUM CANNABIS SATIVA L. EXTRACTS. Czech Republic
2021-02-116 Improvement of acoustic conditions for audio recordings of fish in standard rectangular glass aquariums France
2021-02-117 Carp sperm short-term storage Poland
2021-02-118 Living lymphocytes evaluation for diagnostic purposes, possibilities of replacement of manual observation at different optical magnifications by digital magnification and processing Czech Republic
2021-02-119 Mesenchymal stem cells growth in constrained environment Austria
2021-02-120 Analysis of energetic metabolites in carp spermatozoa during storage Poland
2021-02-121 Investigating the specification of hair cells versus electroreceptors in the lateral line using CRISPR/Cas9 in sterlet UK
2021-02-122 Quantification of selected antibiotic, other pharmaceuticals and pesticides in passive samplers exposed in lake Malawi, Africa UK
2021-03-123 Fabrication, stability and rheological properties of zein/chitosan particles stabilized Pickering emulsions with antioxidant activities of the encapsulated vit-D3. Czech Republic
2021-03-124 Investigation of the physiological mechanisms underpinning pharmaceutical-induced behavioural alterations Sweden
2021-03-125 Influence of ploidy level of different Acipenseridae species on optimal spermatozoa concentration for cryopreservation Czech Republic
2021-03-126 Effect of temperature on food sources are incorporation into fish body Austria
2021-03-127 Burst swimming speed testing in Prussian carp (Carassius gibelio) Czech Republic
2021-03-128 Triploidization in pikeperch (Sander lucioperca) induced by cold shock Czech Republic
2021-03-129 Motility of diplonemids Czech Republic
2021-03-130 Immune response of porcelain disease infected crayfish Croatia
 2021-04-131  Ploidy level analysis in collected samples of the genus Carassius Czech Republic
 2021-04-132  Vizualization of acrosome reaction in sturgeon spermatozoa Poland
 2021-04-133  Pickering emulsion stabilized Andrographolide: A bioactive diet supplimenting growth, digestibility, immunity and viral resistance in Cyprinus carpio. Czech Republic
 2021-04-134  Effect of four different feed formulations on the growth and digestibility of African catfish (Clarias gariepinus) juveniles Czech Republic
 2021-04-135  Fish Mass Estimation in RAS using 3D Camera Czech Republic 
 2021-04-136  Fish individual identification under real conditions Austria 
 2021-04-137  Intermitent respirometry in three cichlids species under normoxic and hypoxic conditions  Czech Republic
 2021-04-138  Factors affecting the prey-predator interactions Czech Republic
2021-04-139 The nerve regeneration in crayfish Czech Republic
2021-04-140 Optimization of mutagenesis by CRISPR/Cas9 in sturgeon USA
2021-04-141 Verification of sex genomic markers in Siberian sturgeon (Acipenser baerii) Poland
2021-04-142 Evaluation of new industrial schooling aquarium Czech Republic
2021-04-143 Pike pearch meat analysis using multispectral camera Czech Republic
2021-04-144 Comparison of performance of new NanoTruth optical microsope with Scanning electron microscope Czech Republic
2021-04-145 Development and testing of basic controls of performance of new NanoTruth optical microscope Czech Republic
2021-04-146 Intermitent respirometry in three cichlids species under normoxic and hypoxic conditions/2 Czech Republic
Implemented projects in 2020
2020
Project no
Name of the project
country of the solver
2020-01-55 Use of humic substances in nutrition of Oncorhynchus mykiss juveniles Germany
2020-01-56 Effects of dietary protein and lipid levels on growth perfromance, body composition, blood biochemistry and ammonia excretion in European grayling (Thymallus thymallus) China
2020-01-57 Combined supplementation of vitamin C and E for attenuating the oxidative damages induced by oxidized fish oil in rainbow trout (Oncorhynchus mykiss) Czech Republic
2020-01-58 Family assignment of broodstock fish of common carp to eliminate inbreeding Czech Republic
2020-01-59 Effect of thermal aclimation on functional response parameters of marbled crayfish (Procambarus virginalis) France
2020-01-60 Predation induces anxiety-like behaviour in crayfish is blocked by pollutants disrupting serotonin pathway UK
2020-01-61 The effect of water oxygen saturation on the performance of pikeperch (Sander lucioperca) in intensive farming Norway
2020-01-62 Antimicrobial peptides in fish seminal plasma and evaluation of their commercial application Malta
2020-01-63 PBS analysis in water samples Austria
2020-02-64 HPLC-MS/MS analysis of pharmaceuticlas and drugs in water samples Slovakia
2020-02-65 PBS analysis in water samples Slovakia
2020-02-66 Study of shark and skate sperm motility Spain
2020-02-67 Effect of cryopreservation on lipid composition of sterlet (Acipenser ruthenus) sperm Czech Republic
2020-02-68 Parentage analysis in sterlet Czech Republic
2020-02-69 Ploidy level analysis in collected samples of the genus Carassius Czech Republic
2020-02-70 The effect of oocyte over-ripening on the ocurrence of spontaneous polyploidy in sterlet. Czech Republic
2020-03-71 Cardiotoxicity of selected pharmaceuticals to early development of nontarget aquatic organisms Croatia
2020-03-72 Use of hormonal preparations in artificial spawning of barbel (Barbu Barbus) Czech Republic
2020-03-73 Does methamphetamine in environmetally relevant concentration affecting ethophysiological parameters of crayfish during early embrional and postembryonal stages Czech Republic
2020-03-74 The cognitive ability of fish during food intake in a new environment after restocking Czech Republic
2020-03-75 Population-specific immune response in percid species Germany
2020-03-76 Determination of Key Parameters in Fish School Behaviour Czech Republic
2020-03-77 Carbon quantum dots for fluorescence labeling of cells Czech Republic
2020-03-78 Measurement of intracellular dynamics after exposition of pesticide Czech Republic
2020-03-80 Fractionlization of stable isotopes in crayfish body Italy
2020-03-81 The role of predator chemical cues in foraging efficiency and intestinal evacuation of the invasive round goby (Neogobius melanostomus) Czech Republic
2020-03-82 Estimation of stocking efficiency of the sterlet juveniles in the Slovakian part of the Danube River Slovakia
2020-03-83 Visible Aquaphotomics on Mineral Water Concentration Hungary
2020-03-84 Bacterial community species specificity and dynamics during storage of fish– microbiological and MALDI TOF approach Czech Republic
2020-03-85 Use of lupine as supplemental feed in carp pond aquaculture Germany
2020-03-86 Population-specific stress response in Eurasian perch (Perca fluviatilis) Czech Republic
2020-03-87 Quantification of selected pharmaceuticals and illicit drugs in extracts from spiders Sweden
2020-03-88 Effect of feed formulation on the diet digestibility and physica properties of feces Czech Republic
2020-04-89 Description of Cobitis hybrid gametogenesis Czech Republic
2020-04-90 Analysis of DNA damage repair in non-model fish species Czech Republic
2020-04-91 AVT correlations with the reproductive condition in male trout Spain
2020-04-92 Application of nanoparticles for stimulation of spermiation in sterlet Czech Republic
2020-04-93 Hydrogels in cell cultures Czech Republic
2020-04-94 Vyhodnocení pokročilé analýzy vzorků vodního prostředí odebraných v souvislosti s rozsáhlou havarijní situací na řece Bečvě za účelem objasnění příčin havárie Czech Republic
2020-04-95 Fish in RAS biomass estimation using new OAK-D camera Czech Republic
2020-04-96 Sea bass identification - non-invasive approach Austria
2020-04-97 Research of herd behaviour of fish in relation to main water contaminants and study of herd behaviour changes ant its aplication for drinking water treatment aparatus. Czech Republic
Implemented projects in 2019
2019
Project NO Name of the project country of the solver
2019-01-01 Analysis of samples from a chronic toxicity test on common carp with synthetic progestins Czech Republic
2019-01-02 Development of the software for cell migration determinaton Czech Republic
2019-01-03 Využití recirkulačních systémů při odchovu parmy obecné (Barbus barbus) za účelem zkrácení odchovného cyklu Czech Republic
2019-01-04 Polarographic assessment of burbot sperm respiration Czech Republic
2019-01-05 Analysis of RNA localization in vitelogenic zebrafish oocytes Czech Republic
2019-01-06 Experimental estimation of gut evacuation rates of invasive alien fish and amphipods Germany
2019-01-07 Evaluation of cardiac activity and histopathological changes of invasive crayfish under hypoxic conditons Czech Republic
2019-01-08 Fish sampling in recirculating aquaculture system (RAS) Czech Republic
2019-01-09 Optimize of the use euryhaline rotifer Brachionus plicatilis for pikeperch Sander lucioperca (L.) larvae first feeding Czech Republic
2019-01-10 Effects of the sub-optimal water temperatures on the feeding activity of pikeperch (Sander lucioperca) with regards to predator’s sex and size of the prey. Hungary
2019-01-11 The effect of interspecific competition on the feeding activity of pikeperch (Sander lucioperca) and prey size preference. Czech Republic
2019-01-12 Use of probiotics for Pikeperch mass production Czech Republic
2019-01-13 Analýza ploidie a obsahu DNA u potomstva experimentálních populací jeseterů Czech Republic
2019-02-14 Estimation of the success of gynogenesis by using microsatellite genotyping Czech Republic
2019-02-15 Ploidy analysis of pikeperch larvae Hungary
2019-02-16 Behaviour study of sexless Danio Czech Republic
2019-02-17 Passive sampler permformance evaluation Czech Republic
2019-02-18 Motility of diplonemids and trypanosomatids Czech Republic
2019-02-19 Analysis of grab water samples from the catchment of the river Odra Czech Republic
2019-02-20 Regeneration of pleopods Italy
2019-02-21 Postembryonic development of the freshwater crayfish Austropotamobius torrentium, Cherax destructor and Procambarus virginalis Czech Republic
2019-02-22 Comparative performance experiment of Silurus glanis strains and families Estonia
2019-02-23 Multiresidual HPLC-MS/MS and in-line SPE-HPLC-MS/MS analysis of drugs, pharmaceuticals and their metabolites in complex environmental samples (waste waters and sludges) Slovakia
2019-02-24 Quatification of selected pharmaceuticals in waste water before and after BDD treatment and method development for identification and semi-quantification of degradation/transformation products after BDD treatment Czech Republic
2019-02-25 Study of pikeperch sperm motility parameters in groups of males with different hormonal stimulation Czech Republic
2019-03-26 Nutrient and digestaility of seven insect meals used in aquaculture Czech Republic
2019-03-27 Passive sampler permformance evaluation Czech Republic
2019-03-28 Evaluation of antiparasitic drugs content in selected fish tissues after treatment Czech Republic
2019-03-29 Development of aquaponic feed - part 1 digestibility Germany
2019-03-30 Development of aquaponic feed - part 2 effect on plant performance Germany
2019-03-31 Screening for hot spots of discharging hormonal activities into surface waters and identification of the effect drivers UK
2019-03-32 Quatification of selected pharmaceuticals in selected tissues from common carp Czech Republic
2019-03-33 Quatification of selected pharmaceuticals and illicit drugs in water samples from pond affected by treated waste water Czech Republic
2019-03-34 The application of immunohistochemical markers on chondrostean and the teleostean tissues Switzerland
2019-03-35 Hyperspectral imaging and Deep learning algorithms for fillet quality assessment of rainbow trout fed insect proteins Italy
2019-03-36 Influence of sperm concentration during freezing on post-thaw outcomes in carp Brazil
2019-03-37 The effect of quinidine on trout sperm motility Czech Republic
2019-03-38 Evaluation of impact Topmouth gudgeon (Pseudorasbora parva) on zooplankton Czech Republic
2019-03-39 Parentage assigment of common carp backup stock for selection experiment Czech Republic
2019-03-40 Quantificaiton of antibiotics from UK rivers Czech Republic
2019-04-41 Diurnal activity in round goby (Neogobius melanostomus) and European bullhead (Cottus gobio) Germany
2019-04-42 Behavioural interactions of invasive round goby (Neogobius melanostomus) and European bullhead (Cottus gobio) Germany
2019-04-43 Invasive crayfish resistance to anoxia evaluated by survival duration and cardiac activity Romania
2019-04-44 The effects garlic (Allium sativum) powder on growth, digestibility, body composition, immunity factors and stress tolerance in perch (Perca fluviatilis) Czech Republic
2019-04-45 Characterization of proteome profile of carp (Cyprinus carpio L) semen during refrigerator storage Czech Republic
2019-04-46 Psychoactive compounds at environmental concentration alter burrowing behavior in the freshwater crayfish Czech Republic
2019-04-47 Biocompatible electrospun nanofibrous supports for deposition of living cells Czech Republic
2019-04-48 Behaviour and fitness in cultured Eurasian perch (Perca fluviatilis) - effect of orgin and domestication Poland
2019-04-49 Response of Desmodesmus quadricaudato selected potentially allelopathic componds Czech Republic
2019-04-50 Dynamic interactions of living cells with the surface of materials Czech Republic
2019-04-51 Collection of datasets for fish identification in 3D Czech Republic
2019-04-52 RNA localization in fish embryos Czech Republic
2019-04-53 Collagen from various mammal species as matrix for tissue engineering Czech Republic
2019-04-54 Fish skin hyperspektral imagery for diests distinqushing Czech Republic

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