Laboratory of Reproductive Physiology
For Students
Courses Taught
Academic and research staff of the laboratory provide teaching for both Czech and international students in the following courses:
- Biology of Cartilaginous Fish
- Fundamentals of Cell Biology
- Reproductive Biology of Fish
- Fish Reproductive Physiology
- Basics of Scientific Communication
Qualification Theses
Our laboratory offers long-term support for Bachelor’s, Master’s, and Ph.D. theses, as well as postdoctoral positions in reproductive physiology. During their studies, students gain hands-on experience with a wide range of laboratory methods, including:
- microscopy (light, video, and high-speed)
- immunocytochemistry and fluorescence microscopy (in particular the observation of ongoing intracellular processes)
- metabolic analyses (e.g., ATP, ADP) and oxidative phosphorylation
- image processing, sperm motility analysis (CASA), and flagellar movement analysis
- assessment of cellular damage caused by reactive oxygen species (ROS), antioxidant enzyme activity, and DNA/RNA integrity
- osmoregulation and cell volume measurements
- artificial fertilization procedures
- gamete cryopreservation
- basics of proteomic and lipidomic analyses
- patch-clamp techniques for studying ion channels and cellular signaling
“We guide students toward precise hypothesis formulation and work with reproducible data.”
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BSc.
Bachelor’s DissertationsRepina Tatiana: Fish sperm osmoresistance as a quality marker during short-term storage. Supervisor: Bondarenko Olga
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Ph.D.
Doctoral ThesesHossain Md. Sabbir: Management of males and sperm of zebrafish (Danio rerio, Hamilton) for practical breeding. Supervisor: Linhart Otomar
Ma Zhijun: How the age of the sperm is imprinted in its RNA. Supervisor: Linhart Otomar
Mohammadi Yaser: Mitochondrial oxidation in fish gametes: comparison to somatic cells. Supervisor: Dzyuba Viktoriya
Morshed Mohammad Riaz: Sperm aging in relation to cryo-resistance in fishes. Supervisor: Dzyuba Borys
Mueni Laurine Mule: Effect of viscosity on fish sperm motility. Supervisor: Boryshpolets Serhii
Rashed Mohammad: Mechanisms of potassium signaling in fish spermatozoa motility. Supervisor: Bondarenko Olga
Syed Awal: Spermatology of endangered, rare and invasive fish species of Central Europe and cryopreservation of their sperm. Supervisor: Rodina Marek
Career Paths of Our Graduates
Throughout its existence, our laboratory has provided a professional and research environment for numerous students who now hold key positions in Czech and international research and educational institutions, including our own institute and faculty. With the consent of our graduates and without indicating academic titles, we may list:
Sayyed Mohammad Hadi Alavi
“Together with my students, we investigate biology of reproduction from basic science to evolutionary, applied and health perspectives in the Reproductive Biology Lab
Miaomiao Xin
“I continue my research on fertility preservation for prepubertal boys and human reprodictive science from the perspective of glycosylation.“
Ping Li
„My major research topics are focusing on the distribution characteristics of pollutants in aquatic environment, the potential toxic effects and molecular mechanisms of pollutants on aquatic organisms, and ecological restoration.“
Yu Cheng
“I am currently engaged in fish breeding research, focusing on disease resistance and growth traits, and exploring the epigenetic mechanisms underlying these processes to promote sustainable aquaculture development. The experience from our lab continues to guide and inspire my research.”