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Business trip report,, Canada, Pech

From October 2023 to July 2024, I completed my PhD internship at the University of Calgary under the supervision of prof. Habibi. During my internship, I carried out two experiments to assess the effects of the fish's anti-progestin gestodene on the reproduction of zebrafish (Danio rerio). My stay at the university greatly benefited me as I learned how to conduct experiments with zebrafish independently and learned several new laboratory techniques. For example, I learned new methods of staining histological sections with their subsequent evaluation, determining plasma hormone levels, RNA extraction with TRIzol or ex vivo tissue culture. In addition, my 9-month stay in Canada helped me to improve my English skills and gain valuable collaboration for the future.

This internship was supported by the Erasmus+ program, the Czech Science Foundation (No. 22-19136S), the Strategic Management Support Program of the University of South Bohemia, the Faculty of Fisheries and Protection of Waters and prof. Habibi. I greatly appreciate all the financial support.

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