Report from the business trip, Hungary, Mahyar Zare

I worked in the laboratory of Fish Immunology, Department of Fish Biology, Research Center for Fisheries and Aquaculture (HAKI), Hungarian University of Agriculture and Life Sciences (MATE), Szarvas, Hungary from 15^th February 2023 to 30^th June 2023. During 136 days internship, I mainly performed sampling tissues including liver, head kidney, and spleen, RNA extraction, making cDNA by reverse transcription, testing RNA integrity by gel electrophoresis, assembling real-time quantitative PCR (qPCR) reactions, qPCR data evaluation, measuring lysozyme enzyme activity and total protein levels from blood plasma. Moreover, I joined one of the lab members to assist the measurement process of the liver antioxidant and intestine digestive enzymes activity. Besides, I attended a workshop to see the isolation of leukocytes from common carp head kidneys and measure the respiratory burst activity and nitric oxide production of the cells. I learned the artificial propagation of European catfish and I updated my knowledge about common carp artificial reproduction. Moreover, I participated in rotifer sampling from common carp rearing ponds. I would like to acknowledge Erasmus+, PPSŘ project, and Laboratory of controlled reproduction and intensive fish culture for financial support.