From 16th November to 31st December 2021, I conducted a business trip to Spain, CENTRO IFAPA AGUA DEL PINO, Cartaya, Huelva focused on the use of PLGA microparticles with mGnRHa in the artificial reproduction of marine fish species.
I visited the Department of Chemistry of Umea University from 3 August to 3 September 2021. The aim of my training activity was acquaintance with the methods and instrumentation used for non-targeted metabolomics. Non-targeted analytical methods are fastly rising field in environmental research. These approaches are closely related to activities and projects running in our laboratory. During my stay, I had an opportunity to collaborate with associate professor Jerker Fick and Annika Johansson from The Swedish Metabolomics Centre. The training activity supported with program Erasmus+ brought me an overview of instrumentation, software, workflows, and quality control applied in another laboratory for non-targeted screening.
The internship was fixed for 3 months at the Lund University, Department of Immunotechnology, LUND, under the supervision of Dr. Fredrik Levander. The internship was aimed at gaining experience in routine molecular techniques as well as advanced proteomics techniques. Initially, I worked on routine tasks and research projects under supervision in the laboratory. I instructed in various methods.
I processed samples designed for three independent proteomics experiments and produced data for publication. In short, experiment number 1- investigates the proteomics changes during nerve regeneration, 2- investigates the proteomics changes in locomotory organ regeneration and 3-Study the gender differences of eye proteome in crayfish.
We visited the Swedish University of Agricultural Sciences in Uppsala from 29th November to 2nd December 2021. During the visit of the Department of Molecular Sciences and Department of Aquatic Sciences and Assessment, we participated in the excursion through analytical laboratories managed by Dr Galia Zamaratskaia, Dr Ali Moazzami, and Dr Oksana Golovko. Within the meetings, we presented the results of our ongoing projects regarding the fate of pharmaceuticals in the environment. Our main aim was to discuss the topics of future cooperation on projects related to the analysis of pollutants in water, soil or tissues of aquatic animals.
The purposed of the trip was to initiate contact between Blue Planet Ecosystems, and Bernard Drosg (BEST). At the same proposed a future collaborations and potential work together on a new Interreg project which will be a follow up project of Algae4FIsh.
In the days of 19th to 21st October 2022 we attended 12th biennial wastewater conference, which took place in Štrbské Pleso in Slovakia. The conference was organized by the Association of Cleaning Experts of the SR (AČE SR). During the conference, we presented ourselves with two lectures (R. Grabic and A. Vojs Staňová) and two posters (G. Fedorova and H. Švecová).
My internship at ICM-CSIC was an inspiring journey enriched by a vibrant research environment. The institute's welcoming spirit was a remarkable experience. Learning new technology applied in animal behavior research provided priceless experiences during my stay at the ICM-CSIC. Acquiring advanced expertise in EthoVision XT, a challenging program for examining the behavior of aquatic animals (Fish and Crustaceans), was one of the highlights of my stay in Barcelona, Spain. This skill has opened new horizons in my understanding of animal behavior.
I gained a deeper understanding of academic concepts by participating in the seminars offered at the ICM-CSIC complex. Additionally, I'm happy to convey that our collaboration with the Renewable Marine Resources laboratory at ICM-CSIC was successful. I learned the matrix system (El Temps and idTracker) software used for circadian rhythm analysis, paving the way for ground-breaking knowledge in these important areas.
I would like to express my sincere appreciation to my laboratory (Laboratory of Freshwater Ecosystems), our faculty (FFPW-RIFCH), and CENAKVA for providing me with this priceless opportunity. The experiences and knowledge gained during the three-month internship have undoubtedly set the course for my future endeavors.
Webpage: https://valeriosbra.wixsite.com/valesbra/people (collaboration)
I have completed a three-month traineeship at the Biotechnical Faculty, University of Ljubljana (BF-UL) from 9th February to 12th May 2023. The internship aimed to gain experience in advanced biotechnical and proteomics techniques. During my stay at BF-UL, I learned several methods for microbial protease activity characterization (phenotypic screening, e.g., “clear zones on agar plates”, azocasein method) and molecular (PCR amplification of selected genes) and correlation of the results collected for selected strains. These skills were applied to evaluate fish or fish products' quality and shelf life in a Food Model (homogenate of common carp meat), and the results were evaluated with professional software. I would like to thank the South Bohemia University project and ERASMUS+ grant for funding my stay in Slovenia.
Between November 12-26, 2021, I conducted a business trip to the IMR - Institute of Marine Research in Bergen (Havforskningsinstituttet), where I worked for the Virgin salmon Project as a postdoc researcher at the Department of Reproduction and Development from 2017 to 2020. The Crispant dnd KO broodstock yet fertile (F0) I created for the Virgin salmon project has finally reached the breeding stage; therefore, germ cells (eggs, sperm) have been sampled for mutational and quality analyses. Also, I fertilized the best parents according to their genotypes. I microinjected dnd mRNA into the fertilized group (F1) to rescue germ cells which carry the sterility trait as dndloss of function mutation. After all the experiments, we evaluated the recent data and upcoming results to be able to present the second paper from this project. We drew a work plan for the further steps and paper submission deadline. All the current results of the project were presented at the department group meeting on November 25th. We have also discussed a new project idea to continue the collaboration and submit the proposal in February 2022 to the Norwegian grant.
I participated in the Max Rubner Centre Opening Symposium for Cardiovascular Diseases 2023 in Berlin, Germany, from 27th to 29th September. Many delegates, scientific researchers, and budding researchers participated in this symposium and shared their knowledge and research findings. I presented my research work entitled “Involvement of apoptosis during in vitro post-ovulatory oocyte ageing in common carp Cyprinus carpio” in the symposium’s poster session. The most interesting part of the conference was knowing the facts about Hans Krebs – the discoverer of the Krebs cycle through his fellow student. I would like to thank the financial support from RVO VURH Laboratore (LIA), which allowed me to participate in this symposium.
June 13th, 2020, I was invited by the Czech Liaison Office for Education & Research (CZELO) to participate in the conference „Benefits and challenges of participation in co-funded partnerships.“ This event was organized by V4 offices. I have presented our experience gained within project BIOCIDE under JPI Water EU program.
The researchers and Ph.D. students from our faculty attended the “12th International Symposium on Reproductive Physiology of Fish” in Crete, Greece, from 15th to 19th May 2023.
This symposium is a continuation of previous excellent meetings of scientific community to promote discussion on the physiology of fish reproduction. Many delegates, scientific researchers, and budding researchers participated in this conference and shared their knowledge and research findings through oral and poster presentations. Here is the list of participants from our faculty.
Oral presentations: Azin Mohagheghi Samarin, Olga Bondarenko, Rigolin Nayak, Linan Gao
Poster presentations: Ievgeniia Gazo, Mujahid Ali Shah, Alexandra Krasilnikova, Gil Joao
The researchers and Ph.D. students from the Laboratory of Reproductive Physiology, Laboratory of Intensive Aquaculture, Laboratory of Germ Cells, and Laboratory of Molecular, Cellular, and Quantitative Genetics attended the “8th International Workshop on the Biology of Fish Gametes” in Gdansk, Poland, from 20th to 23rd September 2022. Many delegates, scientific researchers, and budding researchers participated in this conference and shared their knowledge and research findings in the field of fish gamete biology through oral and poster presentations. The two Ph.D. students of our faculty, M.Sc. Rigolin Nayak, and M.Sc. Essaikiammal Sodalai Muthu Konar were awarded the first prizes in oral and poster presentations. Congratulations to them!
A friendly event called “Student’s Reception” was arranged for all the student participants to discuss their research. An excursion to Malbork castle followed by a Gala dinner and medieval music made the trip more enthusiastic and enjoyable.
Oral presentations: Sergii Boryshpolets, Yu Cheng, Borys Dzyuba, Linan Gao, Ievgeniia Gazo, Vitaliy Kholodnyy, Rigolin Nayak, Rafael Henrique Nobrega, Azadeh Mohagheghi Samarin, Mujahid Ali Shah, Swapnil Gorakh Waghmare
Poster presentations: Sergii Boryshpolets, Vicktorya Dzyuba, Ganna Fedorova, Essaikiammal Sodalai Muthu Konar, Alexandra Krasilnikova, Azin Mohagheghi Samarin, Anatolii Sotnikon, Songpei Zhang
Written by Essaikiammal S.M. Konar and Linan Gao
On 21 Oct 2022, we took part in the official handover of the results of the project "Heritage of the pond landscape - a future UNESCO World Heritage Site?". In this context, I was invited to evaluate the cooperation of our faculty and Austrian partners to date within the INTERREG VA Austria - Czech Republic program and the perspective of continuing the activities of the Algae4fish project. The results achieved during our joint work so far have been highly appreciated by the representatives of the University of Krems and the regional insitutions.
On 8 – 11 May 2023, I was invited by the vice-rector of the University of Crete in Heraklion, prof. Michalis Pavlidis (head of the Dept. of Biology) to give lectures on carp farming ("Principles of common carp rearing and pond aquaculture") as a part of the "Aquaculture, Environment and Society" course. My presentations consisted of four lectures, ending with an exam during this week (examiner Prof. Pavlidis). Department of Biology, which organises the course, mainly focuses on the physiology and welfare of fish and marine hydrobiology, which is why I was asked for a specific section dealing with carp farming. All costs associated with the trip were fully covered by the University of Crete.
On 16-17 August 2023, Dalibor Štys, Renata Štysová Rychtáriková, and Kirill Lonhus visited the ams-OSRAM company in Regensburg at the invitation of dr. Anja Schulz. We presented our quality control microscopes to the development staff, tested the measurement of diode samples and discussed the possibilities of financing joint research.
Written by Renata Štysová Rychtáriková
Scientists and Ph.D. students from the Laboratory of Intensive Aquaculture, the Laboratory of Molecular, Cellular and Quantitative Genetics, the Laboratory of Nutrition, the Laboratory of Controlled Reproduction and Intensive Fish Culture and the Laboratory of Aquatic Toxicology and Ichtyopathology attended the European Aquaculture Society conference in Rimini (Italy) on 27-30 September 2022 on Innovative solutions in a changing world. Many delegates, scientific researchers, and early career researchers attended this conference and shared their knowledge and research findings on innovations and new developments in aquaculture and its components, such as aquaponics and fish nutrition, through oral and poster presentations. Furthermore, a Ph.D. student of our faculty, Mgr. Tomas Pěnka, Ph.D., won a travel award in the European Aquaculture Society (EAS) student section competition.
A great success was the participation of our Colleague MSc. Koushik Roy, Ph.D. who was one of the speakers at Tuesday´s Plenary Session with The move towards circularityas a main topic.
Oral presentations were given by: Oleksandr Malinovskyi, Tomas Pěnka, Carlos Yanes-Roca, Radek Gebauer, Koushik Roy and Mahyar Zare
Poster presentations were given by: Martin Prchal, Martin Kocour, Vojtěch Kašpar, Veronika Piačková, Eliška Zusková, Ehdaa Eltayeb Eltigani Abdelsalam, Deepali Rahi Roy, Alžběta Strouhová, Lenka Kajgrová, Anil Axel Tellbüscher and Hui Jia
Conference participated by: Tomáš Policar, Václav Nebeský
From 11th February to 13th March I completed my internship at the Department of Cell Biology and Histology at the Universidad de Murcia, Spain. During the internship I was introduced to basic techniques in histology and also to the basics of histological image evaluation. The internship was a great benefit for me in terms of professional and personal development. I would like to thank especially Professor Maria Angeles Esteban Abad for her warm welcome and many valuable experiences. The internship was supported by the Erasmus+ programme.
On May 18, 2023, I took part on the international conference Risk Management; climate change and waterways with the presentation "Response of the macrozoobenthos community to extreme flow episodes caused by the climate change" (Z. Adámek, J. Konečná, L. Všetičková, J. Podhrázská, P. Karásek, A. Zajíček). The conference was organized by the Slovak Environmental Agency with the participation of experts from the state administration, scientific and research institutions, water management practice and nature protection organizations.
On September 19-21, 2023, we (Petr Císař, Jan Urban, Mohammad Mehdi Ziaei) are part of the Aquaculture Europe 2023 international conference held in Vienna. All three of us presented contributions in the field of analysis of image and telemetry data from measurements in intensive aquaculture. Petr Císař moderated the section Precision agriculture - Modeling and control systems. Jan Urban gave a paper documenting the data processing carried out as part of the AQUAEXCEL3.0 project.
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